Gene Delivery Using Polymer Therapeutics
Identifieur interne : 001D27 ( Main/Exploration ); précédent : 001D26; suivant : 001D28Gene Delivery Using Polymer Therapeutics
Auteurs : Ernst Wagner [Allemagne] ; Julia Kloeckner [Allemagne]Source :
- Advances in Polymer Science [ 0065-3195 ]
Abstract
Abstract: The use of polymers as synthetic non-viral carriers for introducing nucleic acids into cells appears very appealing. Polymers can be generated in large quantities in chemically defined, non-antigenic and non-immunogenic form. A plethora of different chemical structures and polymer sizes may be applied to tailor-made polymers with optimized characteristics for the extracellular delivery of nucleic acid to the target tissue and the subsequent intracellular delivery into the target cells. For the purpose of nucleic acid transfer, polymers have been applied for incorporating nucleic acids into nanoparticles or microspheres. Alternatively, cationic polymers are applied as carriers for complexing gene vectors into polyplexes. Polyplexes form spontaneously upon mixing negatively charged nucleic acid with the polycationic polymer due to electrostatic interaction. This process can be controlled to result in the formation of particles with defined virus-like sizes which efficiently transfect cell cultures and also have shown encouraging gene transfer potential in in vivo administration. With first-generation polymeric carriers, gene therapeutic effects have been demonstrated in animals, although modest efficiencies and significant toxicity restrict broader therapeutic application. Key issues for future optimization of polyplexes include improved specificity for the target tissue, enhanced intracellular uptake, and reduced toxicity and immunogenicity. Novel cationic polymers have to be made more biocompatible by reducing their potential for unspecific adverse interactions with the host, and by designing them in a biodegradable form. “Smart” polymers and polymer conjugates are being developed that in a dynamic manner present virus-like delivery functions in the appropriate phase of the gene delivery process.
Url:
DOI: 10.1007/12_023
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000F87
- to stream Istex, to step Curation: 000F87
- to stream Istex, to step Checkpoint: 000B95
- to stream Main, to step Merge: 001D39
- to stream Main, to step Curation: 001D27
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Gene Delivery Using Polymer Therapeutics</title>
<author><name sortKey="Wagner, Ernst" sort="Wagner, Ernst" uniqKey="Wagner E" first="Ernst" last="Wagner">Ernst Wagner</name>
</author>
<author><name sortKey="Kloeckner, Julia" sort="Kloeckner, Julia" uniqKey="Kloeckner J" first="Julia" last="Kloeckner">Julia Kloeckner</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:C81D269BC4DAFA249A12B8CE8E2C23AAE10E6433</idno>
<date when="2006" year="2006">2006</date>
<idno type="doi">10.1007/12_023</idno>
<idno type="url">https://api.istex.fr/ark:/67375/HCB-XPMHT571-J/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000F87</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000F87</idno>
<idno type="wicri:Area/Istex/Curation">000F87</idno>
<idno type="wicri:Area/Istex/Checkpoint">000B95</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000B95</idno>
<idno type="wicri:doubleKey">0065-3195:2006:Wagner E:gene:delivery:using</idno>
<idno type="wicri:Area/Main/Merge">001D39</idno>
<idno type="wicri:Area/Main/Curation">001D27</idno>
<idno type="wicri:Area/Main/Exploration">001D27</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Gene Delivery Using Polymer Therapeutics</title>
<author><name sortKey="Wagner, Ernst" sort="Wagner, Ernst" uniqKey="Wagner E" first="Ernst" last="Wagner">Ernst Wagner</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Pharmaceutical Biotechnology, Department of Pharmacy, Butenandtstrasse 5--13, 81377, Munich</wicri:regionArea>
<placeName><region type="land" nuts="1">Bavière</region>
<region type="district" nuts="2">District de Haute-Bavière</region>
<settlement type="city">Munich</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Allemagne</country>
</affiliation>
</author>
<author><name sortKey="Kloeckner, Julia" sort="Kloeckner, Julia" uniqKey="Kloeckner J" first="Julia" last="Kloeckner">Julia Kloeckner</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Pharmaceutical Biotechnology, Department of Pharmacy, Butenandtstrasse 5--13, 81377, Munich</wicri:regionArea>
<placeName><region type="land" nuts="1">Bavière</region>
<region type="district" nuts="2">District de Haute-Bavière</region>
<settlement type="city">Munich</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Allemagne</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="s" type="main" xml:lang="en">Advances in Polymer Science</title>
<title level="s" type="abbrev">Adv Polym Sci</title>
<idno type="ISSN">0065-3195</idno>
<idno type="eISSN">1436-5030</idno>
<idno type="ISSN">0065-3195</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0065-3195</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: The use of polymers as synthetic non-viral carriers for introducing nucleic acids into cells appears very appealing. Polymers can be generated in large quantities in chemically defined, non-antigenic and non-immunogenic form. A plethora of different chemical structures and polymer sizes may be applied to tailor-made polymers with optimized characteristics for the extracellular delivery of nucleic acid to the target tissue and the subsequent intracellular delivery into the target cells. For the purpose of nucleic acid transfer, polymers have been applied for incorporating nucleic acids into nanoparticles or microspheres. Alternatively, cationic polymers are applied as carriers for complexing gene vectors into polyplexes. Polyplexes form spontaneously upon mixing negatively charged nucleic acid with the polycationic polymer due to electrostatic interaction. This process can be controlled to result in the formation of particles with defined virus-like sizes which efficiently transfect cell cultures and also have shown encouraging gene transfer potential in in vivo administration. With first-generation polymeric carriers, gene therapeutic effects have been demonstrated in animals, although modest efficiencies and significant toxicity restrict broader therapeutic application. Key issues for future optimization of polyplexes include improved specificity for the target tissue, enhanced intracellular uptake, and reduced toxicity and immunogenicity. Novel cationic polymers have to be made more biocompatible by reducing their potential for unspecific adverse interactions with the host, and by designing them in a biodegradable form. “Smart” polymers and polymer conjugates are being developed that in a dynamic manner present virus-like delivery functions in the appropriate phase of the gene delivery process.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
</country>
<region><li>Bavière</li>
<li>District de Haute-Bavière</li>
</region>
<settlement><li>Munich</li>
</settlement>
</list>
<tree><country name="Allemagne"><region name="Bavière"><name sortKey="Wagner, Ernst" sort="Wagner, Ernst" uniqKey="Wagner E" first="Ernst" last="Wagner">Ernst Wagner</name>
</region>
<name sortKey="Kloeckner, Julia" sort="Kloeckner, Julia" uniqKey="Kloeckner J" first="Julia" last="Kloeckner">Julia Kloeckner</name>
<name sortKey="Kloeckner, Julia" sort="Kloeckner, Julia" uniqKey="Kloeckner J" first="Julia" last="Kloeckner">Julia Kloeckner</name>
<name sortKey="Wagner, Ernst" sort="Wagner, Ernst" uniqKey="Wagner E" first="Ernst" last="Wagner">Ernst Wagner</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001D27 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001D27 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= ChloroquineV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:C81D269BC4DAFA249A12B8CE8E2C23AAE10E6433 |texte= Gene Delivery Using Polymer Therapeutics }}
This area was generated with Dilib version V0.6.33. |